Monoclonal 1- and 3-Phosphohistidine Antibodies: New Tools to Study Histidine Phosphorylation
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منابع مشابه
Monoclonal 1- and 3-Phosphohistidine Antibodies: New Tools to Study Histidine Phosphorylation
Histidine phosphorylation (pHis) is well studied in bacteria; however, its role in mammalian signaling remains largely unexplored due to the lack of pHis-specific antibodies and the lability of the phosphoramidate (P-N) bond. Both imidazole nitrogens can be phosphorylated, forming 1-phosphohistidine (1-pHis) or 3-phosphohistidine (3-pHis). We have developed monoclonal antibodies (mAbs) that spe...
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An increase in the incidence of histidine phosphorylation of histone H4 in mammalian cells appears to be causally associated with an increase in cellular proliferation (1). Although this and many other reports of histidine phosphorylation in mammalian cells are documented in the literature, little is understood about their biological roles by comparison to hydroxyaminoacid kinases. Many of the ...
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ژورنال
عنوان ژورنال: Cell
سال: 2015
ISSN: 0092-8674
DOI: 10.1016/j.cell.2015.05.046